When iPSC colonies reach approximately 200 cells or over in size, they are ready to be picked and expanded in feeder-free PluriSTEM Human ES/iPSC Media for further experiments. they can indefinitely self-renew in culture and have the potential to differentiate into cell types from all three germ layers i.e. Time course of human iPSC colonies generation using Human Simplicon RNA Reprogramming Kit. (2007) Cell 131 (5): 861–872). The single RNA contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1, and allows for efficient reprogramming … In 2012, Sir John Gurdon and Shinya Yamanaka were honored with the Nobel Prize for “the discovery that mature cells can be reprogrammed to become pluripotent” (Induced PSCs can simply be generated in the lab via introducing stem cell specific transcription factors into primary somatic cells by either viral transduction or non-viral transfection. Our RNA technology has been used across a diverse range of iPSC projects in the research community, from enhancing StemRNA™ is the ideal vector for introducing reprogramming factors into cells, because RNA is:RNA reprogramming is the optimal method for iPSC establishmentAt REPROCELL, our dedicated R&D team are always looking for ways to improve our services. REPROCELL USA Inc. Important note: The user bears the sole responsibility for determining the existence of any third party rights, as well as obtaining any necessary licenses. StemRNA™ is up to 50 times more efficient than any other non-integrative methodology on the market*, allowing you to access the highest quality iPSCs for your research. ✓ Footprint-Free mRNA vectors are rapidly degraded 48 hours following transfection, meaning there is no need to screen your iPSCs for vector retention 2. The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. This kit utilizes Sendai virus particles containing four genes: Oct3/4, Sox2, Klf4, and cMyc. Pluripotent stem cells (PSCs) such as embryonic stem cells (ESC) or induced pluripotent stem cells (iPSC) can give rise to any fetal or adult cell type and have the ability to self-renew indefinitely. Registered office REPROCELL USA Inc., 9000 Virginia Manor Road, Suite 207, Beltsville, 20705, USA, Registered in United StatesPlease click here to see current operating statuses for all REPROCELL offices. StemRNA™ is the ideal vector for introducing reprogramming factors into cells, because RNA is: 1. (2015) Stem Cell Rep 5(4):647-59 The Simplicon™ RNA Reprogramming Technology utilizes a single polycistronic self-replicating RNA strand that is sufficient for generating high numbers of human iPSCs using a single transfection step. Reprogramming is achieved by introducing stem cell-specific transcription factors into a pre-mature or mature somatic cell type.The resulting iPSCs phenotypically and functionally resemble embryonic stem cells (ESCs), e.g. Figure 3. StemRNA - NM Reprogramming kit - next generation of RNA based IPSC reprogramming. We use cookies to deliver the best service to you. (2007) Cell 131 (5): 861–872). At REPROCELL, you can gain access to the most rapid integration-free reprogramming method on the market: StemRNA™ 3rd Gen reprogramming technology. DISCLAIMER: TO THE EXTENT … Due to their ESC-like capabilities, iPSCs are a powerful tool for many developmental or disease-related applications. What is the CytoTune™-iPS Reprogramming Kit?
Our CytoTune-iPS 2.0 Sendai Reprogramming Kits support cutting-edge basic research, and our CTS CytoTune 2.1 Sendai Reprogramming … ✓ Efficient RNA reprogramming is highly efficient, requiring fewer starting target cells tha… CytoTune™-iPS 2.0 Sendai Reprogramming Kit USER GUIDE For efficient, integration-free reprogramming of somatic cells into induced pluripotent stem cells (iPSC) Catalog Numbers A16517, A16518 Publication Number MAN0009378 Revision 2.0. iPSC Reprogramming Kits. As they can be “made” from a person’s own skin or blood, reprogramming can provide a source of patient-specific, genetically identical cells that would not be rejected by the immune system.The raise of iPSCs makes the usage of pluripotent stem cells independent from the availability of embryonic cells and thus independent of ethic controversy and patient-/tissue-matching issues.For human cells, iPSC generation by cellular reprogramming was first described in 2007 by two independent research groups (Yu J et al. Typical reprogramming factors used in different combinations are Oct4, Sox2, Klf4, c-Myc, Nanog and Lin28. The expression of these transcription fac-
any cell type. (2007) Science 318 (5858): 1917–1920; Takahashi K et al. The CytoTune™-iPS Reprogramming Kit is used to reprogram somatic cells into induced pluripotent stem cells (iPSCs). Grab a coffee and check out our free webinars. CytoTune™-iPS Reprogramming Kit 3 pack (3 vials of each gene) A13780-02 1. However, it remains to be assessed in more detail, whether they can be considered fully identical to natural pluripotent cells. For human cells, iPSC generation by cellular reprogramming was first described in 2007 by two independent research groups (Yu J et al.
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