Assays of membrane fusion report either the mixing of membrane lipids or the mixing of the aqueous contents of the fused entities. 2016 Aug;89(8):708-19. doi: 10.1002/cyto.a.22891. Unable to load your collection due to an error 1, and prior to the formation of cell-cell polykaryons, cells joined by small cytoplasmic bridges Mechanisms of membrane fusion can be seen [6]. Related. K Lumley-Sapanski Some theories include: 1. COVID-19 is an emerging, rapidly evolving situation. mediated fusion work? In both virally and chemically mediated cell fusion, therefore, membrane fusion occurs initially between small regions of closely …
Perturbations induced by freeze-fracture preparative procedures, Cowley, A. C., Fuller, N. L., Rand, R. P., and Parsegian, V. A., 1978, Measurement of repulsive forces between charged phospholipid bilayers, d’Avila Nunes, M., 1981, A spin label study of erythrocyte membranes during simulation of freezing, Davidson, R. L., and Gerald, P. S., 1977, Mammalian somatic cell hybridization by polyethylene glycol, in: Eriksson, E., and Albertsson, P. A., 1978, The effect of the lipid composition on the partition of liposomes in aqueous two-phase systems, Fazekas De St. Groth, S., and Scheidegger, D., 1980, Production of monoclonal antibodies: Strategy and tactics, Fuseler, J. W., Miller, C. L., and Fuller, G. M., 1978, Alteration of cytoskeletal morphologies and growth patterns in human fibroblasts treated with PEG, Galfre, G., Howe, S. C., Milstein, C., Butcher, G. W., and Howard, J. C., 1977, Antibodies to major histocompatibility antigens produced by hybrid cell lines, Glasstone, S., and Lewis, D., 1960, Surface chemistry and colloids, in: Hafeti, Y, and Hanstein, W. G., 1974, Destabilization of membranes with chaotropic ions, Hammes, G. G., and Roberts, P. B., 1968, Cooperativity of solvent-macro molecule interactions in aqueous solutions of polyethylene glycol and polyethylene glycol-urea, Hammes, G. G., and Schimmel, P. R., 1967, An investigation of water-urea and water-urea-polyethylene glycol interactions, Hartmann, J. X., Galla, J. D., Emma, D. A., Kao, K. N., and Ganborg, O. L., 1976, The fusion of erythrocytes by treatment with proteolytic enzymes and polyethylene glycol, Herrmann, A., Pratsch, L., Arnold, K., and Lassmann, G., Effect of poly(ethylene glycol) on the polarity of aqueous solutions and on the structure of vesicle membranes, 1983, Honda, K., Maeda, Y, Sasakawa, S., Ohno, H., and Tsuchida, E., 1981, The components contained in polyethylene glycol of commercial grade (PEG-6,000) as cell fusogen, Horwitz, A. F., Michaelson, D., and Klein, M. P., 1973, Magnetic resonance studies on membrane and model membrane systems. Fusion of erythrocytes with polyethylene glycol, Knutton, S., and Bachi, T., 1980, The role of cell swelling and haemolysis in Sendai virus-induced cell fusion and the diffusion of incorporated viral antigens, Knutton, S., and Pasternak, C.A., 1979, The mechanism of cell-cell fusion, Krahling, H., 1981, Discrimination between two fusogenic properties of aqueous polyethylene glycol solutions, Z. Lagerwerff, J. V., Ogata, G., and Eagle, H. E., 1961, Control of osmotic pressure of culture solutions with polyethylene glycol, Larrabee, A. L., 1979, Time-dependent changes in the size distribution of di-stearoylphosphatidylcholine vesicles, Lee, A. G., 1977, Lipid phase transitions and phase diagrams. Fatty acid motions in aqueous lecithin dispersions, Huang, C., and Hui, S. W., 1986, Chemical co-treatments and IMP patching in PEG-induced fusion of turkey and human erythrocytes, Hui, S. W., and Isac, T., Boni, L. T., and Sen, A., 1985, Action of polyethylene glycol on the fusion of human erythrocyte membranes, Hui, S. W., Stewart, T. P., Boni, L. T., and Yeagle, P. L., 1981, Membrane fusion through point defects in bilayers, Ingham, K. C., 1977, Polyethylene glycol in aqueous solution: solvent perturbation and gel filtration studies, Israelachvili, J. N., 1977, Refinement of the fluid-mosaic model of membrane structure, Jain, M. K., and White, H. B., 1978, Long-range order in biomembranes, Johansson, G., 1970, Partition of salts and their effects on partition of proteins in a dextranpoly(ethylene glycol)-water two-phase system, Kao, K. N., and Michayluk, M. R., 1974, A method for high-frequency intergeneric fusion of plant protoplasts, Kleman, M., Williams, C. E., Costello, M. J., and Gulik-Krzywicki, T., 1977, Defect structures in lyotropic smectic phases revealed by freeze-fracture electron microscopy, Knutton, S., 1979, Studies of membrane fusion.
PEG is a key reagent in this protocol. This service is more advanced with JavaScript availablePolyethylene glycol (PEG) has been used frequently as an ideal cell fusogen (Kao and Michayluk, 1974; Davidson and Gerald, 1977; Galfre Over 10 million scientific documents at your fingertips A., 1978, Water and calcium ions in cell fusion induced by poly(ethylene glycol), Boni, L. T., 1983, Ph.D. thesis, State University of New York at Buffalo.Boni, L. T., Hah, J. S., Hui, S. W., Mukherjee, P., Ho, J. T., and Jung, C. y, 1984Boni, L. T., Stewart, T. P., Alderfer, J. L., and Hui, S. W., 1981Boni, L. T., Stewart, T. P., Alderfer, J. L., and Hui, S. W., 1981Boni, L. T., Stewart, T. P., and Hui, S. W., 1984b, Alterations in phospholipid polymorphism by polyethylene glycol, Boss, W. F., and Mort, R. L., 1980, Effects of divalent cations and polyethylene glycol on the membrane fluidity of protoplast, Cabane, B., 1977, Structure of some polymer-detergent aggregates in water, Costello, M. J., and Gulik-Krzywicki, T., 1976, Correlated x-ray diffraction and freeze-fracture studies on membrane model systems. (d) Water-soluble proteins did not diffuse from the erythrocyte interior into the target cell cytoplasm until the PEG was removed.
Study of genetic model organisms has uncovered a unifying principle: cell fusion is a genetically … Cited by. The paired cells are then treated with fusogenic PEG for cell fusion. Fusion is involved in many cellular processes, particularly in There are four fundamental steps in the fusion process, although each of these steps actually represents a complex sequence of events.The exact mechanisms behind this complex sequence of events are still a matter of debate. R A Schlegel Compared with traditional protocols, cell fusion using this approach is well defined with better control, which leads to an improved yield of heterotypic binucleated hybrids. Papers Search for other works by this author on: By continuing to use our website, you are agreeing to (c) Proteins of the erythrocyte membranes were never observed to diffuse into the cultured cell membrane. Fluorescence and freeze-fracture electron microscopy and fluorescence recovery after photobleaching have yielded the following results: (a) In …
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