Issue 052 Issue 084 Issue 082 Issue 042 A fluorescent protein helped researchers illuminate human cellular biology. History on Crystal jellies Calling it a day, he tossed the remnants of his work into a nearby sink. Issue 054 Issue 049 Photoproteins, first isolated from jellyfish several decades ago, are now an integral part of laboratory biology and help researchers do things like mark and identify crucial gene sequences in medical studies. Issue 039 With a nod toward the scientific name of his research animal, Shimomura called the protein aequorin.But if aequorin produced the blue light that Shimomura saw that afternoon, why did the crystal jelly glow green when it was disturbed? Issue 077 Chalfie immediately recognized how GFP could help him. But the original question that got us to GFP wasn’t, “What can it do for us?” Instead, our human curiosity led us to ask, simply, “Why does it glow?” Now, the molecule that answered that basic question helps us illuminate the secrets of human health.Nautilus is a different kind of science magazine. First, they got Then, in 1994, Columbia professor Tulle Hazelrigg, who is married to Chalfie, successfully linked the genes for GFP to another protein while preserving the function of both. Issue 058 But the original question that got us to GFP wasn’t, “What can it do for us?” Instead, our human curiosity led us to ask, simply, “Why does it glow?” Now, the molecule that answered that basic question helps us illuminate the secrets of human health.The Informed Parent: A Science-Based Resource for Your Child’s First Four Years Issue 026 Issue 018 Special Issue Issue 083 Issue 012 Perhaps, Shimomura speculated, another protein was absorbing energy from the blue light and using it to produce green light.
Issue 079 Issue 003 Issue 064 Issue 056 Issue 021 Issue 053 Issue 080 The elusive cells should theoretically be able to...Then, in 1994, Columbia professor Tulle Hazelrigg, who is married to Chalfie, successfully linked the genes for GFP to another protein while preserving the function of both. Issue 033 After Chalfie and Prasher reconnected, Prasher sent along the precious DNA.From there, Chalfie’s team took the first big step toward using GFP as a protein tracer in living cells.
Issue 004 First, they got A few years later, Ghia Euskirchen, a chemical-engineering graduate student with expertise in fluorescence, joined Chalfie’s team, and soon turned up a recent paper on GFP by Prasher.
Shimomura had discovered green fluorescent protein, or GFP. Issue 067 “Jellies are important for humans,” Haddock says. This fusion construct, which Hazelrigg demonstrated in fruit flies, has become one of the most critical applications of GFP in science today. After Chalfie and Prasher reconnected, Prasher sent along the precious DNA.From there, Chalfie’s team took the first big step toward using GFP as a protein tracer in living cells. Suddenly there was what he would later describe as a “bright blue flash.” It didn’t take long to identify the catalyst: The sink where he’d discarded his crystal jelly samples contained seawater draining from nearby tanks, and calcium in the seawater had set the discarded material aglow. Most of them have bodies that are shaped like bells, and they also have various tentacles that are poisonous. Issue 031 Since Something in that sink had set the biological lanterns in the samples alight.He learned that Douglas Prasher, a researcher at Woods Hole Oceanographic Institute at the time, was working on cloning and sequencing GFP—but before they could collaborate, Chalfie went on sabbatical and the researchers lost touch. By then, a lack of funding had led Prasher, who’d been the first person to clone GFP, to quit scientific research altogether.
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